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Product data


Human Myeloperoxidase

Product Human Myeloperoxidase
Product No. 700-03-001
Origin Prepared from human blood that has been shown by certified tests to be negative for HbsAg, HCV and HIV antibodies.
CAS Number 9003-99-0
EC Number 1.11.2.2
Batch No. 270214
MW dimer: 145 000 Da, monomer: 72 500 Da
Storage Stored below 0°C this lyophilized product is stable for years. Storage of the reconstituted form at 4°C for 2 weeks has no effect on enzyme activity. If solutions must be stored for extended periods of time, protein concentration should be kept above 1 mg/ml, and the activity should be determined prior to use.

Test/Analysis Specifications Results
Appearance solid
Purity SDS-PAGE > 98 %
Reinheitszahl A430nm / A280nm > 0.84 (see UV/vis Spectrum)
Solubility Calculated with a molar extinction coefficient of 91.000 M-1cm-1 per hem at 430 nm Reconstitution of 100 µg with 1000 µL distilled water gives approximately a 1.38 µM solution

results of the linear regression
model: y=a0+a1*x
MCD Assay 100 mM Phosphate buffer pH 7
100 mM NaCl
100
M MCD
100
M HOOH
200 nM MPO

One unit of halogenation activity is defined as the amount of enzyme that halogenates 1
mol of MCD min-1 at pH 7.0 and 25 °C.
a1/1=0.35
a1/2=0.32
a1/3=0.34

=0.336 min-1 = 16.9 µM MCD min-1



1.16 Units mg-1
Guaiacol Assay 100 mM Phosphate buffer pH 7
100 µM Guaiacol
100 µM HOOH
50 nM MPO

One unit of peroxidase activity is defined as the amount of enzyme that oxidises 1 µmol of guaiacol min-1 at pH 7.0 and 25 °C.
a1/1=0.224
a1/2=0.220
a1/3=0.218
=0.221 min-1 = 8.3 µM guaiacol min-1


2.31 Units mg-1


Date: January 27th 2016

MPO stock solution was adjusted photometrically and min. 0.116 Units (= 1.16 Units per mg with MCD-Cl-Assay) were filled in each vial prepared for lyophilisation.
Solid lyophilisate per vial contains min. 100 µg active MPO + marginally amounts of salts and impurities (< 1 µg).
 
CAS No: 9003-99-0
EN1
EC 1.11.1.7
Human
Myeloperoxidase
CAS 9003-99-0
MP

 

 


Handling: If you are not fully trained or are unaware of the hazards involved, do not use these compounds! Do not take internally. Wear gloves and mask when handling this product. Avoid contact by all modes of exposure.

Human Eosinophil Peroxidase

 


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