| Product |
Human Myeloperoxidase |
| Product No. |
700-03-001 |
| Origin |
Prepared from human blood that has been shown by certified tests to be negative for HbsAg, HCV and HIV antibodies. |
| CAS Number |
9003-99-0 |
| EC Number |
1.11.2.2 |
| Batch No. |
270214 |
| MW |
dimer: 145 000 Da, monomer: 72 500 Da |
| Storage |
Stored below 0°C this lyophilized product is stable for years. Storage of the reconstituted form at 4°C for 2 weeks has no effect on enzyme activity. If solutions must be stored for extended periods of time, protein concentration should be kept above 1 mg/ml, and the activity should be determined prior to use. |
|
| Test/Analysis |
Specifications |
Results |
| Appearance |
|
solid |
| Purity |
SDS-PAGE |
> 98 % |
| Reinheitszahl |
A430nm / A280nm |
> 0.84 (see UV/vis Spectrum) |
| Solubility |
Calculated with a molar extinction coefficient of 91.000 M-1cm-1 per hem at 430 nm |
Reconstitution of 100 µg with 1000 µL distilled water gives approximately a 1.38 µM solution |
|
|
|
results of the linear regression
model: y=a0+a1*x |
| MCD Assay |
100 mM Phosphate buffer pH 7
100 mM NaCl
100
M MCD
100
M HOOH
200 nM MPO
One unit of halogenation activity is defined as the amount of enzyme that halogenates 1
mol of MCD min-1 at pH 7.0 and 25 °C. |
a1/1=0.35
a1/2=0.32
a1/3=0.34
=0.336 min-1 = 16.9 µM MCD min-1
1.16 Units mg-1
|
| Guaiacol Assay |
100 mM Phosphate buffer pH 7
100 µM Guaiacol
100 µM HOOH
50 nM MPO
One unit of peroxidase activity is defined as the amount of enzyme that oxidises 1 µmol of guaiacol min-1 at pH 7.0 and 25 °C. |
a1/1=0.224
a1/2=0.220
a1/3=0.218
=0.221 min-1 = 8.3 µM guaiacol min-1
2.31 Units mg-1 |
Date: January 27th 2016
MPO stock solution was adjusted photometrically and min. 0.116 Units (= 1.16 Units per mg with MCD-Cl-Assay) were filled in each vial prepared for lyophilisation.
Solid lyophilisate per vial contains min. 100 µg active MPO + marginally amounts of salts and impurities (< 1 µg).
|